Pre-Lab Discussion Questions:
1) What are the four types of blood?
a)
b)
c)
d)
2) Are blood types multiple alleles? Why or why not?
3) Why is it important to know someone’s blood type?
NOTE. All blood and blood sera in this kit are simulated and contain no blood or blood products. These materials cannot be used to type actual human blood. If human anti-sera were available, however, these instructions could be used to type actual human blood.
Materials:
(2 people per group)
1 Hema-tag well plate
One
microscope slide
Two
toothpicks
1. Perform the test
at room temperature. Lay a microscope
slide over the anti-A and anti-B circles on a Hema-Tag. Place 1 drop of anti-A and anti-B in their
respective circles on the microscope slide.
2. Add 1 drop of
cells being tested to each drop of antiserum. (Victim)
3. Mix the
simulated cells and antisera with the appropriate toothpick with their
respective circles, scraping the toothpick firmly against the microscope slide
in the process. Continue stirring for
two minutes or until agglutination is noted.
4. Read the gross
agglutination and record test results.
Note that in this simulation precipitations are recorded as
agglutinations.
POSITIVE: Cells are agglutinated.
NEGATVIE: Cells are not agglutinated.
5. You must
repeat the procedure for each suspect, victim, and the crime scene to determine
who is the MURDERER!!! When you repeat your procedure you must wash the slide
with warm tap-water, blot dry and reuse, following steps 1-4.
1. Victim _______
2. Suspect #1 _______
3. Suspect #2 _______
4. Suspect #3 _______
5. Suspect #4 _______
6. Crime scene _______
Post-Lab
Follow-Up Questions:
1) a) Which simulated blood type(s) showed clumping with the anti-A serum?
b) Which
simulated blood type(s) showed clumping with the anti-B serum?
2) a) If clumping occurs
when both anti-A serum and anti-B serum, what is the blood type?
b) If clumping
does not occur when either anti-A serum and anti-B serum, what is the blood
type?
3) Why is the person with the AB blood type sometimes
called a “universal recipient”?
4) Why is a person with the type O blood unable to receive
blood from any type other than O?
5) Who is the murderer?
How do you know?
6) If you cannot be sure who the murderer is, lay out a
procedure for determining who the murderer is using the anti-Rh serum. Then perform the procedure and show your
results.
7) Identify the
murderer._______________________________________________
Homework:
Use reference materials to research the topic of Rh factors. How were they discovered? How is a person’s
blood tested for Rh factor? Why is this
knowledge important? (250 words,
minimum)